2D GC – TOF Mass Spectrometer

2D-Chromatography with a time of flight mass spectrometer (2D-GC-TOF-MS) is an advanced analytical technique for characterization of samples/compounds with high precision as compared to standard GC-MS systems. 2D GC-MS spectra give increased peak resolution for faster, more confident peak identification and library match.

The system comes with an autosampler to facilitate high-end separation of volatile organic compounds in highly complex mixtures and environmental samples. The two-dimensional output helps in resolving the peak of target analytes even when matrix interferences are present.

Make and Model: Leco, Pegasus 4D with Agilent GC

General Instructions and Guidelines

One sample refers to one injection in any of these modes. Multiple injections of the same sample will be treated as multiple samples.

All the samples will be analyzed on the standard primary column: RXI-5ms, secondary column: RXI-17.

Kindly, note that no injector or column change is not possible for this facility at this stage.

The experimental data provided is only for research/development purposes. These cannot be used as certificates in legal disputes.

A maximum of 3 samples will be analyzed against a single Registration Form.

The analytes expected in the samples should be clearly indicated and MSDS (Material Safety Data Sheet) should be given both for the solvent and the analytes to indicate the toxicity.

Sample Preparation Guidelines:

The sample should be dissolved in a volatile organic solvent preferably hexane or isooctane (HPLC grade, in ppm level concentration.)

Sample volume: Standard injection= 3-5 mL, Method development = 15 mL.

Other solvents like methanol, ethanol, ethyl acetate etc. can also be used.

Solvents such as DMSO, THF and water are not acceptable.

The boiling point of the sample should be below 2800C.

The sample should be free of particulates of size > 0.45 µm

Biomedical samples are not accepted for analysis.

Procedure for Sample Submission

The samples should reach the ULMC along with duly filled Google form along with a copy of payment receipt.

User requisition form for submitting samples

Analysis results: The analysis result which includes the total ion chromatograph and mass spectrum will be emailed to the user.

Getting back the samples: Users desiring to get back the samples need to mark this instruction on the form. Collect them from the lab between 10.00 AM to 11:00 AM within two-three days of completion of the work (official working days only) and uncollected samples will be disposed-off after ten days of intimation of the completion of the work and no reminders will be sent.